Abstract

The aim of the present study was to investigate the prevalence of blaTEM and blaSHV genes in Klebsiella pneumoniae isolates that isolated from 90 hospital’s patients with burns infection in AL-Najaf Governorate – Iraq. The results of the current study demonstrated that out of 90 burns swabs there were 12 isolates were diagnosed as Klebsiella pneumoniae The susceptibility test for 12 isolates of Klebsiella pneumoniae proved that there was highly resistance to antibiotic; their pattern of antimicrobial susceptibility showed that 100% was resistant to amoxicillin , amoxiclav and nitrofurantoin , 83.33% was resistant to cefotaxime , 66.66% was resistant to ceftriaxone, 91.66% was resistant to and ceftazidime, 41.66% was resistant to gentamicin , amikacin , tobramycin and tetracycline. blaTEM and blaSHV genes in multidrug resistant Klebsiella pneumoniae were detected by using monoplex PCR method, the results demonstrated that there were 12 isolate (100 %) was positive for blaTEM gene and 11 isolates (91.66%) was positive for blaSHV gene.