Abstract

laboratories of Biology Department, college of Sciences, which deal with isolation of glucoamylase and optimization of process parameters to achieve maximum yield of glucoamylase by Aspergillus niger . Solid-state fermentation of Aspergillus niger was carried out for enhanced production of glucoamylase (GA) using starch (1%) as the substrate of enzyme maximum activity of the enzyme was achieved under optimum growth conditions . the optimum conditions were the isolated of Aspergillus niger from orange fruits and growth in synthetic medium , it gave high titer of GA activity , the starch as carbon source , yeast extract as nitrogen source , incubation period 96 h , incubation temperature 30 °C and pH = 6