Abstract

This study was conducted in the laboratories of Biology Department,facultyofScience,which deal with isolation,purification and characterization ofof amylase by Escherichia coli which carried out for enhanced production of amylase using starch (1%) asthe substrate of enzyme, the production was carried out by submerged fermentation, the best conditions were the isolated ofamylase in synthetic medium, it gave high titer of amylase activity, the ammonium sulfate as nitrogen source, incubation period 48 h, the starch as carbon source,, incubation temperature 30 °C and pH = 7, The enzyme was purified using ammonium sulphate precipitation(60%) anddialysis, the purified amylase had a maximum activity at pH =7,the amylase was stable with pH values ranging between (7 - 8) and in temperature 30 °C also amylase was stable in (30- 40 ) °C analyses of the amylase for molecular weight was carried out by SDS-PAGE electrophoresiswhich revealed 52 KDa.