Abstract

Summary This study aimed to synthesis of some new 1,2,3,4-Tetrazole derivatives from o-tolidine as a starting materials and measurement of antibacterial activity against two type of pathogenic bacteria (Staphylococcus aureus and Klebsiella pneumoniae) isolated from patients infected with burns infections: The first step includes formation new Schiff bases derivatives from o-tolidine [A] was concentrated with many aromatic aldehydes [4-chlrobenzaldehyde, 4-bromobenzaldehyde, p-dimethyle amino benzaldehyde] in absolute ethanol to give [A1-A3]. The second step includes synthesis [B1-B3] by diazotation of [1-(4-aminophenyl) ethanone, 4-amino-N-(2H-imidazol-2-yl) benzenesulfon amide, 4-nitroaniline], respectively. The formed salt was treated with sodium azide, ethyl acetoacetate and acetylacetone, respectively. [B1-B3] Compounds were entered in 1, 3- dipolar cycloaddition reactions with [A1-A3] in dimethylformamide to give 1, 2, 3, 4-Tetrazole derivatives [AB1-AB3]. Antibacterial activity of nine derivative compounds were done against two types of pathogenic bacteria (p. aeruginosa and S. aureus) the results of this study proved that there were significant bacterial inhibition against p. aeruginosa and S.aureus with inhibition zone 20 mm and 15 mm, respectively.