Abstract

The development of eco-friendly technologies in material synthesis is of considerable importance toexpand their biological applications. A variety of green nanoparticles with well-defined chemicalcomposition, size, and morphology have been synthesized by different methods and their plicationsin many technological areas have been explored. Efficient and green chemistry approaches has led tothe use of microorganisms.Native Lactobacillus of Iraq derived from some local fermented products was used in this study. Total of 15 Lactobacillus isolates were isolated from local ermented food in Najaf city inoculated on deMan, Rogosa and Sharpe (MRS) agar. Plates were incubated for 24 hrs at 30 °C under anaerobiccondition. Titanium nanoparticles had antibacterial activity against indicator strain (Pseudomonas aeruginosa). The efficient isolate (MT14) of Lactobacillus was selected for titanium nanoparticlesformation and identification.Titanium nanoparticles formation by Lactobacillus in some aspects was observed during the study.Characterization of nanoparticles was done by SEM /EDS analysis. Scanning electron Microscoperevealed the formation of spherical nanoparticles with size ranging etween 50-90nm. Energydispersive spectroscopy (EDS) was done using SEM instrument equipped with Bruker EDS attachment showed the optical absorption peak.The efficient isolate (MT14) of Lactobacillus was identified by rphological and biochemicalcharacteristics and confirmed by using species specific primers for the polymerase chain reaction (PCR) that enable specific detection of Lactobacillus rhamnosus. The primers amplify a 122 bp DNAsequence of the genus Lactobacillus rhamnosus.