Abstract

This study aimed to determine the ability of five species from Penicillium include P.chrysogenum ,P.griseofulvum, P.citrinum, P.camembertti and P.arenicola to produce toxin and detect the antibacterial activity of Penicillium sp.on the growth of some pathogenic bacteria include Staphylococcus aureus ,Enterococcus fecalis ,E.coli ,Enterobacter, klebsiella pneumonia ,Proteus mirabilis, Acinetobacter , and Pseudomonas aeruginosa. The mycotoxin extracted by chloroform , while the second method extracted by ethyl acetate,in the first method used chloroform to extraction the toxin from the broth culture in equal volume and used chloroform and methanol 98:2 as mobile phase in isolation the toxin on TLC , in the second method, used ethyl acetate to extraction the toxin from the broth culture in equal volume and used toluene 90%, ethyl acetate and formic acid 1:2:3 as mobile phase in isolation the toxin on TLC .The antibiotic was extracted by used ethyl acetate and used ethyl acetate with acetone 75:25 as the mobile phase to isolation the antibiotic on TLC plate. This study showed penicillium species can produce many types of toxin such as citrinin , patulin, and moniliformin .also antibacterial agent extracted by ethyl acetate and the some active compound produced by P. camemberti and P. griseofulvum and the active compound produced by P. griseofulvum was higher activity in inhibition the growth of bacteria with a diameter of inhibition zone was 23.5 mm , while the active compound produced by P. camemberti gave the diameter of inhibition zone was 11.3 mm.