Abstract

The present study is designed to evaluate the gained some compounds from Pleurotus ostreatus by extracted them and purification by using analytical and preparative HPLC technique, then tested the anticancer activity by invitro crystal violate (C.V) cytotoxicity assay on cancer and normal cell line, then chemical description about type of these compounds by GC-MS technique.Analytical and preparative HPLC carried out with 25%methanol with 1% formic acid mobile phase and description about 12 fractions with retenation time (9,10,11.5,12,12.5,13.5,14.7,15.5,17,18,19.5-21,21.8)respectivily at 254nm.In vitro crystal violate (C.V) cytotoxicity assay on SW-480 colon cancer cell line appeared the excellent inhibition with (1,5,6,7,12) fractions with (1000,500,250,125,62.5 μg/ml), while the same fractions have no effect on vero-101normal cell line.GC-MS analysis data gave us the most probability anticancer compounds in these fractions such as Quinoline, 1H indol, Silane compounds and N-hexadecanoic acid.