Abstract

Abstract : This study was conducted in the laboratories of Biology Department, college of Sciences, which deal with isolation of glucoamylase and optimization of process parameters to achieve maximum yield of glucoamylase by Aspergillus niger . Solidstate fermentation of Aspergillus niger was carried out for enhanced production of glucoamylase (GA) using starch (1%) as the substrate of enzyme maximum activity of the enzyme was achieved under optimum growth conditions . the optimum conditions were the isolated of Aspergillus niger from orange fruits and growth in synthetic medium , it gave high titer of GA activity , the starch as carbon source , yeast extract as nitrogen source , incubation period 96 h , incubation temperature 30 °C and pH = 6