الخلاصة

Background: DNA fingerprint ,genetic relationship were determined for ten Iraqi maize genotypes. Materials: DNA was extracted from apical fresh leaves using Genomic DNA mini Kit and ten ISSR markers were examined , amplification conducted using Thermo cycler and products examined using agarose gel electrophoresis .Results: ISSR markers amplification product ranging in their molecular size between 114bp and 1098bp producing 95 polymorphic out of 152 main band. Primers UBC808, UBC809, UBC812 , UBC814, UBC816, 844A, UBC807 and HB12 success in giving a unique fingerprint for most studied genotypes with high value of unique bands reached to five in primer UBC809. The higher value for polymorphism ,efficiency and discriminatory value were 80,0.25 and 17.89 in primers UBC808,UBC811 and UBC816 respectively while the lower value for these three parameters were 31.25 , 0.052 and 5.26 respectively produced by primer HB12. Values of genetic distance and genotypes arrangement in phyllogenetic tree in clusters and sub clusters not strongly concerned with genotypes pedigree or collection sites . Conclusion : ISSR markers could efficiently used for fingerprinting and genetic relationships, in future this would facilitate breeding programs for heterosis.