الخلاصة

Objective: Many materials types were used for the biomolecules interaction including insoluble organic compounds to use them in the biosensor or in vivo. The aim of the present study is to immobilize the prolactin hormone on the surface of two new synthesized Schiff bases (LH1 &LH2) and to study the different thermodynamic parameters of the adsorption processes. Methods: Different concentrations of the prolactin solutions were incubated with aliquots of 50mg of LH1 or LH2 and the quantity of the prolactin adsorbed were calculated. The experiments were repeated at different temperatures to calculate the thermodynamic parameters. Results: There are significant adsorption of prolactin molecules on the surfaces of LH1 & LH2, 97ng/mg and 48ng/mg, respectively. The adsorption process obeyed Freundlich adsorption isotherm indicating heterogeneity of the surface of the compounds or different forces of interaction involved in the adsorption between prolactin and the compounds. Thermodynamic analysis indicated an exothermic and spontaneous adsorption process of the prolactin on the surfaces of LH1 & LH2. The percentage of the quantities of prolactin desorbed from the surface of compound were 7.8% from LH1 and 11.2% from LH2 surfaces indicating a stronger forces of interaction between prolactin molecules and LH1. Conclusion: It can be concluded that the prolactin molecules can be immobilized on the surface of LH1 with good quantity of adsorption and low desorption ensuring the ability to use LH1 as a surface for in vivo or in vitro applications. Keywords: Azo, adsorption, Protein, Prolactin, and Desorption.