Molecular characterization of coagulase negative staphylococci isolates from clinical and carries specimens

Abstract

Sixty coagulase-negative staphylococci (CONS) isolates collected from patients and carries   (forty of clinical origin and twenty of carries origin) during the period from October 2012 to March 2013. Isolates diagnosed depending on the morphological criteria cultural and biochemical test and Vitek-2 system test. The most common isolates species among was S.epidermidis which was accounted for 18 isolates (30%), S.saprophyticus was the second with 13 isolates (21.7%), followed by S.hemolyticus was 11 isolates (18.3%), S.homins was 7 isolates (11.7%), S.lentus was 6 isolates (10%), S.capitis was 4 isolates (6.7%) and one isolated (1.6%) of S.auricularis. multiple PCR was used to detection the mecA&icaA genes . the result indicated 30 isolates (75%) from all 40 clinical isolates produce mecA gene while 7 isolates (35%) produce this gene in carries isolates. the icaA gene produce in 12 (30%) clinical isolates while two  isolates (10%) have ability this gene in carries isolates. Ten clinical isolates (25%) produce mecA&icaA genes while only one carries isolate (5%) produce two genes.